GPC S-19 Analysis of Foodstuffs Modular Multiple Analytical Method for the Determination of Pesticide Residues in Foodstuffs
1 Aim and scope
This official method specifies a procedure for the residue
analysis of organochlorine, organophosphorus, nitrogencontaining
and other pesticides in foodstuffs. It reflects
the DFG Multiresidue Method S 19 in its original [1] as
well as in its modified version [2] and affords a much
broader field of application.
2 Definition
The term pesticide residue content of a foodstuff is defined
as the content determined by using this method. It
is expressed in mg/kg.
3 Introduction
The Multiresidue Method S 19 of the DFG Manual including
the Cleanup Method 6 (gel chromatographic
cleanup) [3] has proved to be used successfully in many
laboratories because of its broad applicability for the gas
chromatographic determination of pesticide residues in
foodstuffs. It was also included into the respective European
Standards [4, 5].
In the meantime a modification of the extraction and partition
step has been implemented [2]. It requires less experimental
efforts and manages without dichloromethane
which is undesirable for toxicological and ecological reasons.
As the results from validation studies demonstrate,
this modification has the same broad field of application
as the original DFG Method S 19, and it is included in
the German Collection of Official Methods as Method L
00.00-34.
In both cases the entire method consists of four stages:
extraction and partition, gel permeation chromatography
(GPC), mini silica gel column chromatography, and gas
chromatographic determination. Except for the central
GPC, several variations occur on each stage depending
on the kind of the sample material and the residues to be
analyzed. They can be combined with each other in a variety
of ways according to the requirements.
This is difficult to realize in the references published previously.
However, every laboratory analyzing pesticide
residues is constrained to provide a detailed description
of the specific analytical conditions used together with its
results. This is easier to see when the individual stages
are presented just as “modules” which can be listed in
the particular test report. At the same time this facilitates
the course of work in the residue laboratory, when it can
be clearly specified with which modules an individual
sample has to be processed. If the analyst, however, introduces
any deviations, this should be recorded in the
test report.
请点击此处下载
收藏
