GPC EPA3640A净化方法

仪器资料 · 发表于 2018-7-15 18:33:46

GPC EPA3640A净化方法
1.1 Gel-permeation chromatography (GPC) is a size exclusion cleanup
procedure using organic solvents and hydrophobic gels in the separation of
synthetic macromolecules (1). The packing gel is porous and is characterized by
the range or uniformity (exclusion range) of that pore size. In the choice of
gels, the exclusion range must be larger than the molecular size of the molecules
to be separated (2). A cross-linked divinylbenzene-styrene copolymer (SX-3 Bio
Beads or equivalent) is specified for this method.
1.2 General cleanup application - GPC is recommended for the elimination
from the sample of lipids, polymers, copolymers, proteins, natural resins and
polymers, cellular components, viruses, steroids, and dispersed high-molecularweight
compounds (2). GPC is appropriate for both polar and non-polar analytes,
therefore, it can be effectively used to cleanup extracts containing a broad
range of analytes.
1.3 Specific application - This method includes guidance for cleanup of
sample extracts containing the following analytes from the RCRA Appendix VIII and
Appendix IX lists:

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