《High-Performance gradient elution》LLOYD R. SNYDER，JOHN W. DOLAN

仪器资料 · 发表于 2019-3-9 17:33:10

《High-Performance gradient elution》The Practical Application of the Linear-Solvent-Strength Model

《High-Performance gradient elution》LLOYD R. SNYDER，JOHN W. DOLAN- 一起下吧

1 INTRODUCTION 1
1.1 The “General Elution Problem” and the Need for Gradient Elution 1
1.2 Other Reasons for the Use of Gradient Elution 4
1.3 Gradient Shape 7
1.4 Similarity of Isocratic and Gradient Elution 10
1.4.1 Gradient and Isocratic Elution Compared 10
1.4.2 The Linear-Solvent-Strength Model 13
1.5 Computer Simulation 18
1.6 Sample Classification 19
1.6.1 Sample Compounds of Related Structure
(“Regular Samples”) 19
1.6.2 Sample Compounds of Unrelated Structure
(“Irregular” Samples) 19
2 GRADIENT ELUTION FUNDAMENTALS 23
2.1 Isocratic Separation 23
2.1.1 Retention 23
2.1.2 Peak Width and Plate Number 24
2.1.3 Resolution 25
2.1.4 Role of Separation Conditions 27
2.1.4.1 Optimizing Retention [Term a of Equation (2.7)] 27
2.1.4.2 Optimizing Selectivity a [Term b of Equation (2.7)] 28
2.1.4.3 Optimizing the Column Plate Number N [Term c of
Equation (2.7)] 28
2.2 Gradient Separation 31
2.2.1 Retention 32
2.2.1.1 Gradient and Isocratic Separation Compared for
“Corresponding” Conditions 34
2.2.2 Peak Width 38
2.2.3 Resolution 39
2.2.3.1 Resolution as a Function of Values of S for Two Adjacent
Peaks (“Irregular” Samples) 42
2.2.3.2 Using Gradient Elution to Predict Isocratic Separation 45
2.2.4 Sample Complexity and Peak Capacity 47
2.3 Effect of Gradient Conditions on Separation 49
2.3.1 Gradient Steepness b: Change in Gradient Time 50
2.3.2 Gradient Steepness b: Change in Column Length or Diameter 51
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