Waters UPLC超高效液相色谱初学者手册（英文）

仪器资料 · 发表于 2017-7-20 15:11:48

In 2004, separation science was revolutionized with the introduction of Ultra-Performance Liquid
Chromatography [UPLC® Technology]. Significant advances in instrumentation and column technology
were made to achieve dramatic increases in resolution, speed and sensitivity in liquid chromatography.
For the first time, a holistic approach involving simultaneous innovations in particle technology and
instrument design was endeavored to meet and overcome the challenges of the analytical laboratory.
This was done in order to make analytical scientists more successful and businesses more profitable
and productive.
For more than four decades, reducing stationary-phase particle size has been exploited to improve
chromatographic separation efficiency. Until recently, LC technology had reached a plateau in which the
benefits of reducing particle size could not be fully realized due to the negative influence of instrument
band spreading and limited pressure range.
The ACQUITY UPLC System [Figure 1] has removed those barriers, enabling columns packed with smaller
particles [1.7 – 1.8 μm] to reach their theoretical performance, while precisely delivering mobile phase at
pressures up to 1030 bar [15,000 psi], thus providing a new level of chromatographic performance.
UPLC Technology facilitates improvements of resolution, sensitivity and speed to be achieved, without
compromise. Whether the separation goal is to achieve ultra-fast analysis, increase throughput while maintaining resolution, improving resolution while decreasing analysis time or achieve ultra-high resolution, the flexibility of the ACQUITY UPLC System enables method requirements to be met [Figure 2].