电子书：《Liquid Chromatography–Mass Spectrometry》
In this book, I have tried to show the way in which high performance liquid
chromatography–mass spectrometry (LC–MS) has developed, somewhat slowly
it has to be said, into a powerful hybrid analytical technique.
In the first chapter, I have discussed the limitations of high performance liquid
chromatography (HPLC) and mass spectrometry when used in isolation and how
the combination of the two allows these to be overcome. In this chapter, the
effect of combining the two techniques with regard to the individual performance
characteristics are explored.
In Chapters 2 and 3, brief descriptions of HPLC and MS are provided. These
are not fully comprehensive but are intended to provide a brief description of
those aspects of each of the techniques which are pertinent to a consideration of
LC–MS.
Seven different LC–MS interfaces are described in Chapter 4, with particular
emphasis being placed on their advantages and disadvantages and the ways in
which the interface overcomes (or fails to overcome) the incompatibilities of the
two techniques. The earlier interfaces are included for historical reasons only
as, for example, the moving-belt and direct-liquid-introduction interfaces, are not
currently in routine use. The final chapter (Chapter 5) is devoted to a number of
illustrative examples of the way in which LC–MS has been used to solve various
analytical problems.
I have tried to make it clear that the LC–MS combination is usually more
powerful that either of the individual techniques in isolation and that a holistic
approach must be taken to the development of methodologies to provide data
from which the required analytical information may be obtained. Data analysis is
of crucial importance in this respect and for this reason the computer processing
of LC–MS data is considered in some detail in both Chapters 3 and 5.
LC–MS is still not used in many laboratories where it would be a cost-effective
investment. In order that interested readers can gauge whether they should ‘test
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xii Liquid Chromatography–Mass Spectrometry
the water’, a number of applications which illustrate the range of analyses and
the analytical performance that may be obtained from modern LC–MS interfaces
have been described. Although your precise application may not appear here, I
hope that the descriptions are general enough for the reader to draw parallels
with their own work.
Bob Ardrey
University of Huddersfield, UK
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