2008-Electrophoresis-扫描温度梯度聚焦毛细管电泳测定火星物种的氨基酸

仪器资料 · 发表于 2018-6-26 17:27:47

2008-Electrophoresis-扫描温度梯度聚焦毛细管电泳测定火星物种的氨基酸
Scanning temperature gradient focusing (TGF) is a recently described technique for the
simultaneous concentration and separation of charged analytes. It allows for high analyte
peak capacities and low LODs in microcolumn electrophoretic separations. In this paper,
we present the application of scanning TGF for chiral separations of amino acids. Using a
mixture of seven carboxyfluorescein succinimidyl ester-labeled amino acids (including five
chiral amino acids) which constitute the Mars7 standard, we show that scanning TGF is a
very simple and efficient method for chiral separations. The modulation of TGF separation
parameters (temperature window, pressure scan rate, temperature range, and chiral selector
concentration) allows optimization of peak efficiencies and analyte resolutions. The use
of hydroxypropyl-b-CD at low concentration (1–5 mmol/L) as a chiral selector, with an appropriate
pressure scan rate ( 20.25 Pa/s) and with a low temperature range (3–257C over
1 cm) provided high resolution between enantiomers (Rs .1.5 for each pair of enantiomers)
using a short, 4 cm long capillary.With these new results, the scanning TGF method
appears to be a viable method for in situ trace biomarker analysis for future missions to
Mars or other solar system bodies.

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