ÄKTA™ pure
User Manual
1 Introduction ...................................................................................................................................................... 6
1.1 Important user information ............................................................................................................................................ 7
1.2 ÄKTA pure overview ........................................................................................................................................................... 9
1.3 ÄKTA pure user documentation .................................................................................................................................... 11
2 The ÄKTA pure instrument ............................................................................................................................. 13
2.1 Overview illustrations ........................................................................................................................................................ 14
2.2 Liquid flow path ................................................................................................................................................................... 25
2.3 Instrument control panel ................................................................................................................................................. 27
2.4 Instrument modules ........................................................................................................................................................... 32
2.4.1 System pumps ...................................................................................................................................................................................................... 33
2.4.2 Mixer ......................................................................................................................................................................................................................... 37
2.4.3 Valves, overview .................................................................................................................................................................................................. 38
2.4.4 Inlet valves ............................................................................................................................................................................................................. 39
2.4.5 Mixer valve ............................................................................................................................................................................................................. 46
2.4.6 Injection valve ....................................................................................................................................................................................................... 49
2.4.7 Loop valve .............................................................................................................................................................................................................. 52
2.4.8 Column valves ...................................................................................................................................................................................................... 55
2.4.9 Versatile valve ....................................................................................................................................................................................................... 59
2.4.10 pH valve ................................................................................................................................................................................................................... 60
2.4.11 Outlet valves .......................................................................................................................................................................................................... 64
2.4.12 Pressure monitors ............................................................................................................................................................................................... 67
2.4.13 UV monitors ........................................................................................................................................................................................................... 69
2.4.14 Conductivity monitor ......................................................................................................................................................................................... 72
2.4.15 Flow restrictor ....................................................................................................................................................................................................... 74
2.5 Installation of internal modules .................................................................................................................................... 76
2.6 Accessories ............................................................................................................................................................................ 79
3 ÄKTA pure external modules .......................................................................................................................... 86
3.1 External air sensors ............................................................................................................................................................ 87
3.2 Fraction collector F9-C ..................................................................................................................................................... 89
3.2.1 Function .................................................................................................................................................................................................................. 90
3.2.2 Fraction collector F9-C illustrations ............................................................................................................................................................ 92
3.2.3 Cassettes, Cassette tray and racks ............................................................................................................................................................. 96
3.2.4 Connect tubing to the ÄKTA pure instrument ......................................................................................................................................... 102
3.3 Fraction collector F9-R .................................................................................................................................................... 103
3.4 Sample pump S9 and S9H .............................................................................................................................................. 107
3.5 I/O-box E9 ............................................................................................................................................................................... 111
3.5.1 Overview of the I/O-box ................................................................................................................................................................................... 112
3.5.2 Analog connector and signals ....................................................................................................................................................................... 114
3.5.3 Digital connector and signals ........................................................................................................................................................................ 116
3.5.4 Connect external equipment to the I/O-box ........................................................................................................................................... 118
3.6 Connection of external modules .................................................................................................................................. 121
4 System configuration ...................................................................................................................................... 123
4.1 Configuration overview .................................................................................................................................................... 124
4.2 Configure modules ............................................................................................................................................................. 130
4.2.1 Configuration of inlet valves .......................................................................................................................................................................... 131
4.2.2 Configuration of Mixer valves ........................................................................................................................................................................ 132
4.2.3 Configuration of Loop valves ......................................................................................................................................................................... 133
4.2.4 Configuration of column valves .................................................................................................................................................................... 134
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