Confirmation of Peanut Protein Using Peptide Markers in Dark ChocolateUsing lcms

仪器资料 · 发表于 2018-9-4 06:37:20

Confirmation of Peanut Protein Using Peptide Markers in Dark Chocolate Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
Detection of peptides from the peanut allergen Ara h 1 by liquid chromatography-mass spectrometry
(LC-MS) was used to identify and estimate total peanut protein levels in dark chocolate. A comparison
of enzymatic digestion subsequent to and following extraction of Ara h 1 from the food matrix revealed
better limits of detection (LOD) for the pre-extraction digestion (20 ppm) than for the postextraction
digestion (50 ppm). Evaluation of LC-MS instruments and scan modes showed the LOD could be
further reduced to 10 ppm via a triple-quadrupole and multiple-reaction monitoring. Improvements in
extraction techniques combined with an increase in the amount of chocolate extracted (1 g) improved
the LOD to 2 ppm of peanut protein. This method provides an unambiguous means of confirming the
presence of the peanut protein in foods using peptide markers from a major allergen, Ara h 1, and
can easily be modified to detect other food allergens.

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