LC/MS A Practical User’s Guide(液质联用 入门手册)
作者:MARVIN C. MCMASTER
A JOHN WILEY & SONS, INC., PUBLICATION
Copyright 2005 by John Wiley & Sons, Inc. All rights reserved.
Preface xi
1 Introduction to LC/MS 1
1.1 Why LC/MS?, 1
1.2 Molecular Weights and Structure Studies, 4
1.3 LC/MS Systems, 4
1.4 System Costs, 7
1.5 Competitive Systems, 7
2 The HPLC System 9
2.1 HPLC System Components, 9
2.2 Gradient versus Isocratic Systems, 14
2.3 Micro HPLC Systems, 16
2.4 HPLC Tubing and Fittings, 18
3 The HPLC Column and Separation Modes 21
3.1 Column Construction, 21
3.2 Column Packing Materials, 23
3.3 Normal-Phase Columns, 25
3.4 Other Bonded-Phase Silica Columns, 26
3.5 Optimizing Reverse-Phase Column Use, 28
3.6 Silica Ion-Exchange Columns, 30
3.7 Silica Size-Separation Columns, 31
……