Simple Method IS-MRM to Monitor Lysophospholipidsand Phospholipids During LC-MS

Simple Method (IS-MRM) to Monitor Lysophospholipidsand Phospholipids During LC-MS Method Development via In-Source CID Overview 􀂾hospholipids and lysophospholipidsyield matrix effects in LC-MS 􀂾These lipids present at very high levels in plasma samples after protein precipitation 􀂾We developed a simple method, "In-source Multiple Reaction Monitoring" (IS-MRM), to monitor their elution using m/z184 (Q1) to 184 (Q3) at low collision cell energy 􀂾4-5 minute methods developed to separate basic drugs analytesfrom these lipids 􀂾Unexpected results indicate the concentrations of these lipids become steady-state with time if not eluted from column gx8z|8[/pan]