Simple Method IS-MRM to Monitor Lysophospholipidsand Phospholipids During LC-MS

仪器资料 · 发表于 2019-2-22 07:06:11

Simple Method (IS-MRM) to Monitor Lysophospholipidsand Phospholipids During LC-MS Method Development via In-Source CID
Overview
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hospholipids and lysophospholipidsyield matrix effects in LC-MS
􀂾These lipids present at very high levels in plasma samples after protein precipitation
􀂾We developed a simple method, "In-source Multiple Reaction Monitoring" (IS-MRM), to monitor their elution using m/z184 (Q1) to 184 (Q3) at low collision cell energy
􀂾4-5 minute methods developed to separate basic drugs analytesfrom these lipids
􀂾Unexpected results indicate the concentrations of these lipids become steady-state with time if not eluted from column
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