反相HPLC流动相的PH选择

仪器资料 · 发表于 2018-7-24 23:32:19

反相HPLC流动相的PH选择
Selecting the proper buffer pH is
necessary to reproducibly separate
ionizable compounds by reversed-phase
liquid chromatography. Selecting an
improper pH for ionizable analytes often
leads to asymmetric peaks that are broad,
tail, split, or shoulder. Sharp, symmetrical
peaks are necessary in quantitative
analysis in order to achieve low detection
limits, low relative standard deviations
(RSD) between injections, and
reproducible retention times. We will
discuss when buffering is needed, how to
select buffer pH, and how to choose
the proper buffer system for that pH.

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