Mass spectrometry based functional proteomics from molecular machines to

仪器资料 · 发表于 2019-1-30 13:43:48

Mass spectrometry based functional proteomics from molecular machines to protein networks
The study of protein-protein interactions by mass spectrometry is an increasingly
important part of post-genomics strategies to understand protein function. A variety of
mass spectrometry–based approaches allow characterization of cellular protein assemblies
under near-physiological conditions and subsequent assignment of individual proteins
to specific molecular machines, pathways and networks, according to an increasing
level of organizational complexity. An appropriate analytical strategy can be individually
tailoredfrom an in-depth analysis of single complexes to a large-scale characterization
of entire molecular pathways or even an analysis of the molecular organization of entire
expressed proteomes. Here we review different options regarding protein-complex
purification strategies, mass spectrometry analysis and bioinformatic methods according
to the specific question that is being addressed.

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