用LC-MS-MS非SPE法同时检测动物组织中三聚氰胺和三聚氰酸

仪器资料 · 发表于 2017-7-20 15:08:12

LC-MS-MS非SPE法同时检测动物组织中三聚氰胺和三聚氰酸LC-MS-MS simultaneous determination of Melamine and Cyanuric Acid in animal tissues

J. Qiu, Y. Zhang, H. X. Yu, G. Chen, X. F. Mao, X. Y. Tang, S. M. Yang*
Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of
Agricultural Sciences, Beijing 100081, China

An optimized method was developed for simultaneous determination of melamine (MEL) and
cyanuric Acid (CYA) in meat, liver and kidney tissues of five kinds of animals (cattle, goat, pig,
chicken and fish). MEL and CYA were extracted with acetonitrile/water (70/30) by shaking.
After being simply defatted with n-hexane, both analytes were detected within 12 min using
liquid chromatography-triple quadrupole mass spectrometry (LC/MS/MS) on Waters Atlantis
HILIC columns. The optimized method using isotope-labeled analytes as internal standard was
linear over a range of concentrations with 2-500 ng/mL for MEL and 4-500 ng/mL for CYA. The
mean recoveries of MEL and CYA in tissues respectively ranged from 83-108% and 75-102%
with RSD < 11%. The limits of detection (LOD) for MEL and CYA respectively were 0.01 and
0.02 mg/g of tissues. The results showed that the optimized method have a simple procedure of
sample preparation, a wide linear range and higher sensitivity, and therefore suit for
determination of MEL and CYA in animal tissues especially when analyzing big batch of
samples from pharmacokinetic studies of MEL and CYA in animal.

请点击此处下载

请先注册会员后在进行下载

已注册会员，请先登录后下载