Light Scattering, Size Exclusion Chromatography and Asymmetric Flow Field Flow

仪器资料 · 发表于 2019-4-10 16:02:19

Light Scattering, Size Exclusion Chromatography and Asymmetric Flow Field Flow Fractionation
GPC新著——光散射、尺寸排除色谱和非对称场流分离（英文版）
Powerful Tools for the Characterization
of Polymers, Proteins and Nanoparticles
Stepan Podzimek

A John Wiley & Sons, Inc., Publication
Light Scattering, Size Exclusion Chromatography and Asymmetric Flow Field Flow- 一起下吧

Light Scattering, Size Exclusion Chromatography and Asymmetric Flow Field Flow- 一起下吧

Preface ix
1 Polymers 1
1.1 Introduction 1
1.2 Molecular Structure of Polymers 2
1.2.1 Macromolecules in Dilute Solution 4
1.3 Molar Mass Distribution 10
1.3.1 Description of Molar Mass Distribution 13
1.3.1.1 Distribution Functions 17
1.3.1.2 Molar Mass Averages 21
1.4 Methods for the Determination of Molar Mass 23
1.4.1 Method of End Groups 23
1.4.2 Osmometry 24
1.4.2.1 Vapor Pressure Osmometry 24
1.4.2.2 Membrane Osmometry 25
1.4.3 Dilute Solution Viscometry 26
1.4.3.1 Properties of Mark-Houwink Exponent 30
1.4.3.2 Molecular Size from Intrinsic Viscosity 31
1.4.3.3 Dependence of Intrinsic Viscosity on Polymer Structure,
Temperature, and Solvent 33
1.4.4 Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass
Spectrometry 34
1.4.5 Analytical Ultracentrifugation 35
1.5 Keynotes 36
1.6 References 36
2 Light Scattering
2.2 Types of Light Scattering 39
2.2.1 Static Light Scattering 40
2.2.1.1 Particle Scattering Functions 47
2.2.1.2 Light Scattering Formalisms 54
2.2.1.3 Processing the Experimental Data 54
2.2.2 Dynamic Light Scattering 59
2.3 Light Scattering Instrumentation 63
2.4 Specific Refractive Index Increment 65
2.5 Light Scattering in Batch and Chromatography Mode 72
2.6 Parameters Affecting Accuracy of Molar Mass Determined by Light
Scattering 78
2.7 Examples of Light Scattering Measurement in Batch Mode 84
2.8 Keynotes 96
2.9 References 97
3 Size Exclusion Chromatography
3.1 Introduction 99
3.2 Separation Mechanisms 102
3.2.1 Steric Exclusion 102
3.2.2 Restricted Diffusion 103
3.2.3 Separation by Flow 103
3.2.4 Peak Broadening and Separation Efficiency 105
3.2.5 Secondary Separation Mechanisms 113
3.3 Instrumentation 114
3.3.1 Solvents 118
3.3.2 Columns and Column Packing 122
3.3.3 Detectors 127
3.3.3.1 UV Detector 130
3.3.3.2 Refractive Index Detector 131
3.3.3.3 Infrared Detector 133
3.3.3.4 Evaporative Light Scattering Detector 134
3.3.3.5 Viscosity Detector 135
3.3.3.6 Light Scattering Detector 140
3.3.3.7 Other Types of Detectors 142
3.4 Column Calibration 143
3.4.1 Universal Calibration 149
3.4.2 Flow Marker 152
3.5 SEC Measurements and Data Processing 154
3.5.1 Sample Preparation 154
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