安捷伦液相色谱-G6500 系列四极杆-飞行时间质谱联用仪现场培训手册

仪器资料 · 发表于 2019-5-15 20:23:40

版本 ......................................................................................................................................................................... 2
目录 ......................................................................................................................................................................... 3
1. 基本原理 ............................................................................................................................................................... 6
1.1 QUAD四极杆的基本原理 ........................................................................................................................................ 6
1.2 TOF的基本原理 ...................................................................................................................................................... 7
1.3 Q-TOF的工作方式 .................................................................................................................................................. 8
1.4 离子源技术和原理 ................................................................................................................................................. 9
1.4.1 ESI源—大气压电喷雾离子源 ......................................................................................................................... 9
1.4.2 APCI源—大气压化学电离离子源 ................................................................................................................... 9
1.4.3 ESI with Agilent Jet Steam Technology—带有安捷伦喷射流技术的ESI源 .................................................. 10
1.5不同离子源的比较 ............................................................................................................................................... 10
2 硬件概览 ..............................................................................................................................................................11
2.1典型配置图 ........................................................................................................................................................... 11
2.2仪器序列号位置 ................................................................................................................................................... 11
2.3系统结构图示意图 ............................................................................................................................................... 12
3 开机和关机 ...........................................................................................................................................................13
3.1 开机步骤 ............................................................................................................................................................... 13
3.2关机步骤 ............................................................................................................................................................... 17
4 软件概览和仪器快捷控制功能 ..............................................................................................................................19
4.1 桌面常用软件图标介绍 ....................................................................................................................................... 19
4.2 软件功能区、状态区及快捷方式介绍 ................................................................................................................ 20
4.3 Q-TOF的快捷控制功能 ........................................................................................................................................ 22
5 调谐和校正 ..........................................................................................................................................................29
5.1配制调谐液 ........................................................................................................................................................... 29
5.2 自动调谐介绍和比较 ........................................................................................................................................... 31
5.2.1 Instrument State ............................................................................................................................................ 31
5.2.2 System tune .................................................................................................................................................... 31
5.2.3 Transmission tune .......................................................................................................................................... 33
5.2.3 Mass calibration/Check .................................................................................................................................. 33
5.2.4 Preferences ..................................................................................................................................................... 34
5.3 保存调谐结果，返回采集界面 ............................................................................................................................ 35
5.4 调谐文件的选择 ................................................................................................................................................... 35
6 方法编辑 ..............................................................................................................................................................36
6.1 PROPERTIES ............................................................................................................................................................... 36
6.2 Q-TOF采集参数的设定 ........................................................................................................................................ 36
6.2.1 时间段和实验段: ........................................................................................................................................... 42
6.2.2 General标签 .................................................................................................................................................. 43
6.2.4 Q-TOF采集参数的设定 ................................................................................................................................ 45
6.2.5 Ref Mass ........................................................................................................................................................ 52
6.2.6 Chromatogram ............................................................................................................................................... 53
6.3 保存方法 ............................................................................................................................................................... 53
7 单针样品和工作列表的编辑和运行 .....................................................................................................................54
7.1 运行单针样品 ....................................................................................................................................................... 54
7.2编辑和运行WORKLIST ............................................................................................................................................ 55
7.2.1 创建工作列表 ................................................................................................................................................ 55
7.2.2 往工作列表添加一个样品 ............................................................................................................................. 56 一起下吧
7.2.3 往工作列表添加多个样品 ............................................................................................................................. 56
7.2.4 设置工作列表完成后自动待机 ..................................................................................................................... 57
7.2.5 保存和运行工作列表 .................................................................................................................................... 57
8 定性分析 ..............................................................................................................................................................58
8.1 定性软件的主要功能区和常用快捷键 ................................................................................................................ 58
8.2 放大或缩小谱图 ................................................................................................................................................... 59
8.3 提取色谱图 ........................................................................................................................................................... 60
8.4 提取质谱图 ........................................................................................................................................................... 61
8.5 提取和扣除质谱背景 ........................................................................................................................................... 61
8.6 改变质谱图离子显示的阈值 ............................................................................................................................... 63
8.7 轮廓图转化为棒状图 .......................................................................................................................................... 63
8.8 提取紫外色谱图和光谱图 ................................................................................................................................... 63
8.9 WALK CHROMATOGRAM和SPECTRUM PREVIEW............................................................................................................ 64
8.10 计算信噪比 ........................................................................................................................................................ 65
8.11 积分 .................................................................................................................................................................... 65
8.12 打印分析报告 ..................................................................................................................................................... 66
8.13 计算分子式 ......................................................................................................................................................... 67
8.14 FORMULA CALCULATOR ............................................................................................................................................. 68
8.15 MASS CALCULATOR .................................................................................................................................................. 68
8.16 TARGET MS/MS数据分析 ..................................................................................................................................... 69
9 如何建立PCDL数据库 ..........................................................................................................................................71
9.1 建立基本的PCD数据库....................................................................................................................................... 71
9.2 通过结构简式找到化合物 ................................................................................................................................... 73
9.3 通过化合物产生结构式 ....................................................................................................................................... 78
9.4 SEND SPECTRA TO PCDL .............................................................................................................................................. 80
9.5 PCDL谱库检索 ...................................................................................................................................................... 82
10 定量方法的建立 ..................................................................................................................................................85
10.1 生成CEF文件....................................................................................................................................................... 85
10.2 新建数据分析批 ................................................................................................................................................. 86
10.3 方法编辑 ............................................................................................................................................................. 87
10.4 打印报告 ............................................................................................................................................................. 94
11 仪器的日常维护 ..................................................................................................................................................96
11.1 MS维护周期表 .................................................................................................................................................. 96
11.2 清洗维护雾化器组件（NEBULIZER） ................................................................................................................... 96
11.3 每天清洗电喷雾雾化室 ..................................................................................................................................... 96
11.4 每周清洗电喷雾雾化室 ..................................................................................................................................... 98
11.5 卸下电喷雾雾化器 ............................................................................................................................................. 99
11.6 调整电喷雾雾化器针位置 ............................................................................................................................... 100
11.7 机械泵的日常维护 ........................................................................................................................................... 102
11.8 检查废液桶 ....................................................................................................................................................... 102
11.9 氮气发生器的维护 ........................................................................................................................................... 103
11.10 更换液氮时的注意事项 ................................................................................................................................. 103
附录 ....................................................................................................................................................................... 104
附录1 离子源参数快速参考指南 ............................................................................................................................ 106
附录1.1 电喷雾源 (ESI) ........................................................................................................................................ 106
附录1.2带有Agilent喷射流技术的ESI源(Jet Stream) ..................................................................................... 106
附录1.3 大气压化学电离源（APCI） ................................................................................................................. 106
附录2 不同调谐液离子列表 ................................................................................................................................... 107
附录3 毛细管的维护 ............................................................................................................................................... 108
附录3.1 导电毛细管的清洗步骤，备件号G1960-80060 .................................................................................. 108 一起下吧
附录3.2 绝缘透明毛细管的清洗步骤，备件号59987-20040 ........................................................................... 109
附录4 质谱常用消耗品........................................................................................................................................... 110
附录5 仪器配置INSTRUMENT CONFIGURATION .............................................................................................................. 111
附录6 推荐学习资料 ............................................................................................................................................... 112
书籍 ...................................................................................................................................................................... 112
期刊 ...................................................................................................................................................................... 112
网站 ...................................................................................................................................................................... 112