HPLC分析常见药物的方法集
1. Vitamins
Oil-soluble vitamins................................................................................................................................ 21
Vitamin A fatty acid ester.........................................................................................................................21
Retinol................................................................................................................................................. 21
Carotene..............................................................................................................................................21
β-Carotene ..........................................................................................................................................22
Vitamin D ............................................................................................................................................22
Tocopherols .................................................................................................................................... 23,24
Tocopherol and tocopherol acetate .....................................................................................................24,25
Vitamin K............................................................................................................................................. 25
A drink including vitamins ....................................................................................................................... 26
Water-soluble vitamins ....................................................................................................... 25,26,27,28,29
Allantoin and water-soluble vitamins ..........................................................................................................29
Thiamine and its modifide compounds ...................................................................................................... 30
Riboflavin and related compounds ........................................................................................................... 30
Nicotinic acid and derivatives................................................................................................................. 30
Pantothenyl alcohol ............................................................................................................................... 30
Coenzyme A and derivatives .................................................................................................................. 31
L-Ascorbic acid and erythorbic acid ......................................................................................................... 31
2,4-Dinitrophenylhydrazone of L-ascorbic acid ........................................................................................... 31
2. Carbohydrates
Monosaccharides and disaccharides.........................................................................................................32
Monosaccharides ................................................................................................................................. 32
Unsaturated chondro-disaccharides .......................................................................................................... 33
Sugar alcohols ..................................................................................................................................... 33
Oligosaccharides ........................................................................................................................33,34,35
Fructooligosaccharides........................................................................................................................... 34
Oligosaccharides of soft drink ................................................................................................................. 34
Maltooligosaccharides ..................................................................................................................35,36,37
Cyclodextrins ...................................................................................................................................37,38
Pyridylamino(PA)-Sugar chains..............................................................................................................38,39
Polydextrose..........................................................................................................................................40
Pullulans ..........................................................................................................................................40,41
Chondroitin...........................................................................................................................................41
Chondroitin sulfate A ..............................................................................................................................41
Chondroitin sulfate B ..............................................................................................................................41
Chondroitin sulfate C ..............................................................................................................................42
Hyaluronic acid .....................................................................................................................................42
Heparin................................................................................................................................................42
Heparan sulfate .....................................................................................................................................42
Keratan sulfate.......................................................................................................................................43
Keratan polysulfate .................................................................................................................................43
Nucleosides and bases...........................................................................................................................43
Nucleic acid bases ................................................................................................................................43
Orotic acid ...........................................................................................................................................44
Nucleotides ................................................................................................................................44,45,46
Oligonucleotides....................................................................................................................................46
Poly(A) .................................................................................................................................................46
Sugar nucleotides..............................................................................................................................46,47
Plasmid pBR322 restriction fragments.........................................................................................................47
4. Amino acids, Peptides, Proteins
Amino acids..........................................................................................................................................48
Taurine in nutritious drink..........................................................................................................................48
PTC-amino acids....................................................................................................................................48
PTH-amino acids ....................................................................................................................................48
NBD-amino acids...................................................................................................................................49
Peptides and proteins..............................................................................................................................49
Glutathione, oxidized and reduced form ....................................................................................................49
Angiotensins.....................................................................................................................................49,50
Insulins ............................................................................................................................................50,51
Peptides ......................................................................................................................51,52,53,55,56,59
Human immunoglobulins.....................................................................................................................53,54
Normal Human IgA................................................................................................................................54
Cytochrome c........................................................................................................................................55
Peptide fragments from myoglobin.............................................................................................................55
Glycine oligomers ..................................................................................................................................56
Casein .................................................................................................................................................56
Proteins for molecular weight marker................................................................................................56,57,58
Proteins ................................................................................................................................................58
Human IgM(κ) myeloma protein ...............................................................................................................54
5. Organic acids, Fatty acid derivatives
Organic acids .......................................................................................................................................60
Pyruvic acids .........................................................................................................................................60
Unsaturated fatty acid methyl esters ...........................................................................................................61
Methyl linolenate and methyl γ-linolenate ....................................................................................................61
Triacylglycerols ......................................................................................................................................61
Food preservatives ............................................................................................................................62,67
p-Hydroxybenzoic acid esters..............................................................................................................62,63
EDTA-chelates ........................................................................................................................................63
Antioxidants ................................................................................................................................63,64,65
Color additives ......................................................................................................................................65
Annatto extract ......................................................................................................................................66
Aspartame in a soft drink....................................................................................................................66,67
Stevioside and rebaudioside A .................................................................................................................67
Glycyrrhizic acid....................................................................................................................................67
7. Natural products
Purine Alkaloids .....................................................................................................................................68
Alkaloids ..............................................................................................................................................68
Chlorophyll a and b ...............................................................................................................................68
Crude Drugs (Glycosides) ........................................................................................................................69
Paeoniflorin...........................................................................................................................................69
Paeoniflorin in Toki-shakuyaku-san..............................................................................................................70
Saponins from roots of rubus suavissimus S.Lee............................................................................................70
Saikosaponins .......................................................................................................................................70
Ginsenosides ........................................................................................................................................71
Flavonoids .......................................................................................................................................71,72
Baicalin................................................................................................................................................72
Flavanone glycosides.........................................................................................................................72,73
Isoflavones from miso, fermented soybean paste ..........................................................................................73
Catechins ........................................................................................................................................73,74
2-Phenylethyl glycosides ..........................................................................................................................74
Sennosides ......................................................................................................................................74,75
Arbutin and related compounds ................................................................................................................75
Paeonol................................................................................................................................................76
Aflatoxins..............................................................................................................................................76
8. Steroids
Steroids...........................................................................................................................................77,80
Adrenocorticosteroids...............................................................................................................77,78,79,80
Hydrocortisone and prednisone ................................................................................................................78
Androgens ............................................................................................................................................81
Synthetic androgens ..........................................................................................................................81,82
Estrogens and progestins .........................................................................................................................82
Estrogens .........................................................................................................................................82,83
Norgestrel and ethinylestradiol .................................................................................................................83
Phenacyl esters of bile acids from pig gall ..................................................................................................83
Ecdysones.............................................................................................................................................84
Sterols ..................................................................................................................................................84
Cardiac glycosides.................................................................................................................................85
Steroids from toad venom ........................................................................................................................86
9. Drugs, Metabolites
Pain-killing drugs ...............................................................................................................................87,90
Indometacin ..........................................................................................................................................87
A cough/cold medication........................................................................................................................87
Ingredients in a cough/cold medication ................................................................................................87,88
Analgesics ............................................................................................................................................88
Benzethonium chloride ............................................................................................................................89
Benzalkonium chloride ............................................................................................................................89
Ingredients in drug for rhinitis....................................................................................................................89
Ingredients in an eye drop .......................................................................................................................89
Codeine ...............................................................................................................................................90
Collunarium...........................................................................................................................................90
Naphazoline hydrochloride and chlorpheniramine maleate............................................................................90
Histamine antagonists .............................................................................................................................91
Acidic non-steroidal anti-inflammatory drugs ...........................................................................................91,93
Antipsychotic drugs.................................................................................................................................92
Antiepileptics .........................................................................................................................................92
Tricyclic antidepressants...........................................................................................................................93
Calcium antagonists................................................................................................................................94
Hypolipidemic drugs...............................................................................................................................95
Lovastatin..............................................................................................................................................95
Antiarrhythmic drugs...........................................................................................................................94,97
Local anesthetics ....................................................................................................................................97
Diuretics ..........................................................................................................................................96,97
Antineoplastic and related compounds .......................................................................................................96
Folic acid and its antagonists ...................................................................................................................96
Nicotinic acid analogues..................................................................................................................98,100
Basic compounds ...................................................................................................................................98
Disinfectants ..........................................................................................................................................98
Acyclovir ..............................................................................................................................................99
Carnitine chloride...................................................................................................................................99
Xanthine oxidase inhibitor and related compounds .......................................................................................99
Anti-human immunodeficiency virus (HIV) agents .........................................................................................100
Acidic and Basic drugs .........................................................................................................................101
Chlorhexidine ......................................................................................................................................101
Purine catabolites .................................................................................................................................102
Catecholamines ...................................................................................................................................102
Hippuric and methylhippuric acids....................................................................................................101,102
Antibacterial agents .............................................................................................................................104
Sulfonamides ........................................................................................................................103,104,105
Antibiotics ..........................................................................................................................................105
Nalidixic acid antibiotics ...............................................................................................................105,106
Cephalosporin antibiotics..........................................................................................106,107,108,109,110
Cefoxitin sodium...........................................................................................................................111,112
Penicillin antibiotics ..............................................................................................................................111
Amoxicillin..........................................................................................................................................111
Cephalosporin C.................................................................................................................................112
Ceftriaxone ........................................................................................................................................112
Erythromycin antibiotic drugs..................................................................................................................113
Tetracycline antibiotics ..........................................................................................................................113
Vancomycin ........................................................................................................................................113
11. Pesticides, Environmental
Copper 8-quinolinolate .........................................................................................................................114
Herbicides .........................................................................................................................................114
Atrazine derivatives..............................................................................................................................114
Triazine herbicides ........................................................................................................................114,115
Urea herbicides...................................................................................................................................115
Acid amide herbicides .........................................................................................................................115
Carbamate herbicides ..........................................................................................................................115
Carbamate insecticides ........................................................................................................................116
Fungicides..........................................................................................................................................116
Imazalil..............................................................................................................................................116
2,4-Dinitrophenylhydrazones of aldehydes and ketones ..............................................................................117
Polyaromatic hydrocarbons.............................................................................................................117,118
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