色谱柱和色谱系统的故障课件

仪器资料 · 发表于 2017-9-27 16:09:53

色谱柱和色谱系统的故障课件

Determining the Cause and Correcting High Back Pressure 查出原因纠正压力
Check pressure with/without column - many pressure problems are due to blockages in the system or guard
有／无柱子时检查压力  －  许多压力问题是由于系统或保护柱的阻塞
If Column Pressure is high 如果柱压高:
Wash column - Eliminate column contamination and
冲洗柱子          plugged packing  排除柱污染和填料堵塞
                  - high molecular weight/adsorbed
                     compounds  高分子量／被吸附化合物
                  - precipitate from sample or buffer
                     样品或缓冲液中的沉淀物
Back flush column - Clear plugged frit 反冲柱子－清洁阻塞的滤头
Change frit - Clear plugged frit 更换滤头