安捷伦液相色谱- G6200系列飞行时间质谱联用仪

仪器资料 · 发表于 2017-12-10 14:20:24

目录
版本 .......................................................................................................................................................................... 2
目录 .......................................................................................................................................................................... 3
1 基本原理 ................................................................................................................................................................ 6
1.1 TOF的基本原理 ....................................................................................................................................................... 6
1.2 离子源技术和原理 .................................................................................................................................................. 7
1.3.1 ESI源—大气压电喷雾离子源 ......................................................................................................................... 7
1.3.2 APCI源—大气压化学电离离子源 ................................................................................................................... 7
1.3.3 ESI with Agilent Jet Steam Technology—带有安捷伦喷射流技术的ESI源 ................................................... 8
1.3不同离子源的比较 .................................................................................................................................................. 8
2 硬件概览 ................................................................................................................................................................ 9
2.1典型配置图 .............................................................................................................................................................. 9
2.2仪器序列号位置 ...................................................................................................................................................... 9
2.3系统结构图示意图 ................................................................................................................................................ 10
3 开机和关机 ............................................................................................................................................................11
3.1 开机步骤 ................................................................................................................................................................ 11
2关机步骤 ................................................................................................................................................................... 14
4 软件概览 ................................................................................................................................................................15
4.1 桌面常用软件图标介绍 ........................................................................................................................................ 15
4.3 G1367/G4226自动进样器的快捷控制功能 ......................................................................................................... 18
4.4 二元泵的快捷控制功能 ........................................................................................................................................ 20
4.5 柱温箱的快捷控制功能 ........................................................................................................................................ 21
4.6 DAD的快捷控制功能 ............................................................................................................................................ 21
4.7 VWD的快捷控制功能 ........................................................................................................................................... 22
4.8 TOF的快捷控制功能 ............................................................................................................................................. 22
5 调谐和校正 ...........................................................................................................................................................23
5.1配制调谐液 ............................................................................................................................................................ 23
5.2 自动调谐介绍和比较 ............................................................................................................................................ 24
5.3 调谐界面功能概述 ................................................................................................................................................ 24
5.3.1 Manual Tune ................................................................................................................................................... 24
5.3.2 Diagnostics ...................................................................................................................................................... 25
5.3.3 Instrument State ............................................................................................................................................. 25
5.3.4 Preferences ..................................................................................................................................................... 25
5.4 INITIAL TUNE .............................................................................................................................................................. 26
5.5 STANDARD TUNE ......................................................................................................................................................... 27
5.6 QUICK TUNE ............................................................................................................................................................... 27
5.7 CHECK TUNE ............................................................................................................................................................... 27
5.8 CALIBRATION .............................................................................................................................................................. 28
5.9 保存调谐结果，返回采集界面 ............................................................................................................................ 29
6 方法编辑 ...............................................................................................................................................................30
6.1 PROPERTIES ................................................................................................................................................................ 30
6.2自动进样器的参数设置 ........................................................................................................................................ 30
6.2.1 微盘自动进样器的参数设置（G1367、G1377、G4226） ......................................................................... 30
6.2.2 标准自动进样器的参数设置（G1329） ...................................................................................................... 31
6.3泵的参数设置 ........................................................................................................................................................ 32
6.3.1 二元泵的参数设置 ......................................................................................................................................... 32
6.3.2 四元泵的参数设置 ......................................................................................................................................... 33 一起下吧：www.yqdaw.com
安捷伦 HPLC- 6200 系列 TOF 液质联用仪现场培训手册 Page 4
6.4 柱温箱参数设置 .................................................................................................................................................... 34
6.5 紫外检测器的参数设置 ........................................................................................................................................ 34
6.5.1 DAD检测器的参数设置 ................................................................................................................................ 34
6.5.2 VWD检测器的参数设置 ............................................................................................................................... 35
6.6 TOF采集参数的设定 ............................................................................................................................................. 36
6.6.1 时间段和实验段: ............................................................................................................................................ 36
6.6.2 General标签 ................................................................................................................................................... 36
6.6.3 源参数设置标签 ............................................................................................................................................. 37
6.6.2.1 Dual ESI源的参数设置 .............................................................................................................................................37
6.6.2.2 ESI with Agilent Jet Stream Technology的参数设置 ...............................................................................................38
6.6.2.3 APCI源的参数设置 ...................................................................................................................................................38
6.6.4采集参数的设定 ............................................................................................................................................ 39
6.6.5 Ref Mass ......................................................................................................................................................... 39
6.6.6 Chromatogram ................................................................................................................................................ 40
6.7 保存方法 ................................................................................................................................................................ 40
7 单针样品和工作列表的编辑和运行 .....................................................................................................................41
7.1 运行单针样品 ........................................................................................................................................................ 41
7.2编辑和运行WORKLIST ............................................................................................................................................. 42
7.2.1 创建工作列表 ................................................................................................................................................. 42
7.2.2 往工作列表添加一个样品 ............................................................................................................................. 43
7.2.3 往工作列表添加多个样品 ............................................................................................................................. 43
7.2.4 设置工作列表完成后自动待机 ..................................................................................................................... 44
7.2.5 保存和运行工作列表 ..................................................................................................................................... 44
8 定性分析 ...............................................................................................................................................................45
8.1 定性软件的主要功能区和常用快捷键 ................................................................................................................ 45
8.2 放大或缩小谱图 .................................................................................................................................................... 46
8.3 提取色谱图 ............................................................................................................................................................ 46
8.4 提取质谱图 ............................................................................................................................................................ 47
8.5 提取和扣除质谱背景 ............................................................................................................................................ 48
8.6 改变质谱图离子显示的阈值 ................................................................................................................................ 49
8.7 轮廓图转化为棒状图 ........................................................................................................................................... 49
8.8 提取紫外色谱图和光谱图 .................................................................................................................................... 49
8.9 WALK CHROMATOGRAM和SPECTRUM PREVIEW............................................................................................................ 50
8.10 计算信噪比 ......................................................................................................................................................... 51
8.11 积分 ..................................................................................................................................................................... 51
8.12 打印分析报告 ...................................................................................................................................................... 52
8.13 计算分子式 .......................................................................................................................................................... 53
8.14 FORMULA CALCULATOR .............................................................................................................................................. 54
8.15 MASS CALCULATOR ................................................................................................................................................... 54
9 仪器的日常维护 .....................................................................................................................................................55
9.1 MS维护周期表 ..................................................................................................................................................... 55
9.2 清洗维护雾化器组件（NEBULIZER） ..................................................................................................................... 55
9.3 每天清洗电喷雾雾化室 ........................................................................................................................................ 55
9.4 每周清洗电喷雾雾化室 ........................................................................................................................................ 57
9.5 卸下电喷雾雾化器 ................................................................................................................................................ 58
9.6 调整电喷雾雾化器针位置 .................................................................................................................................... 59
9.7 机械泵的日常维护 ................................................................................................................................................ 60
9.8 检查废液桶 ............................................................................................................................................................ 60
9.9 氮气发生器的维护 ................................................................................................................................................ 61
9.10 更换液氮时的注意事项 ...................................................................................................................................... 61
附录 ..........................................................................................................................................................................62 一起下吧：www.yqdaw.com
安捷伦 HPLC- 6200 系列 TOF 液质联用仪现场培训手册 Page 5
附录1 离子源参数快速参考指南 .............................................................................................................................. 62
附录1.1 电喷雾源 (ESI)........................................................................................................................................... 62
附录1.2带有Agilent喷射流技术的ESI源(Jet Stream) ....................................................................................... 62
附录1.3 大气压化学电离源（APCI） ................................................................................................................... 62
附录2 不同调谐液离子列表 ...................................................................................................................................... 63
附录3 毛细管的维护 .................................................................................................................................................. 64
附录3.1 导电毛细管的清洗步骤，备件号G1960-80060 .................................................................................... 64
附录3.2 绝缘透明毛细管的清洗步骤，备件号59987-20040 ............................................................................. 65
附录4 更换质谱切换阀内的滤芯 .............................................................................................................................. 66
附录5 1100/1200/1260系列液相色谱仪常用消耗品 .............................................................................................. 67
附录6 1290系列液相色谱仪常用消耗品 ................................................................................................................. 68
附录7 质谱常用消耗品.............................................................................................................................................. 69
附录8 仪器配置INSTRUMENT CONFIGURATION ................................................................................................................ 70
附录9 推荐学习资料 .................................................................................................................................................. 73
书籍 ......................................................................................................................................................................... 73
期刊 ......................................................................................................................................................................... 73
网站 ......................................................................................................................................................................... 73