Intact mass analysis of monoclonal antibodies by capillaryelectrophoresis—Mass

仪器资料 · 发表于 2018-5-4 15:08:35

Intact mass analysis of monoclonal antibodies by capillaryelectrophoresis—Mass spectrometry
tCharacterization of monoclonal antibody (mAb) therapeutics by intact mass analysis provides importantinformation on sequence integrity and post-translational modifications. In order to obtain domain specificinformation, monoclonal antibodies are reduced to heavy and light chain components or enzymaticallydigested into smaller portions or peptides. Liquid chromatography (LC) is widely used for separation ofthe antibody fragments in line with mass spectrometry (MS) for characterization. Capillary electrophore-sis (CE) is an analytical technique with high separation efficiency, high sensitivity, and minimal inter-runsample carryover. Combining the resolving power of CE with electrospray ionization (ESI) MS has greatpotential in regards to accurate mass characterization of protein therapeutics and has been a long sought-after approach. However, the intrinsic technical difficulty in coupling CE to MS has hindered the broadapplication of CE-MS across the biopharmaceutical industry. Recently, a CE-MS interface has been devel-oped [1] and commercialized. Herein, we report implementation of this technology for coupling CE to anAgilent time-of-flight (TOF) mass spectrometer. CE-MS provides an attractive complement to LC–MS forseparation and intact mass determination of mAbs and antibody-based therapeutics.

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