DETERMINATION OF ARISTOLOCHIC ACID IN TRADITIONAL CHINESE MEDICINES

仪器资料 · 发表于 2018-5-7 17:26:34

DETERMINATION OF ARISTOLOCHIC ACID IN TRADITIONAL CHINESE
MEDICINES AND DIETARY SUPPLEMENTS

INTRODUCTION
Aristolochic acid is a known nephrotoxin and potential carcinogen that can be found in
Aristolochia species (spp.), Bruguntia spp. or Asurum spp. The Aristolochiu spp. are
most often found in traditional Chinese medicines. Because of the similarity of Chinese
names for several herbs and because of the Chinese tradition of the interchangeability of
similarly named herbs, the possibility exists for the inadvertent substitution of innocuous
herbs with Aristolochiu spp. not only in traditional medicines but also in botanicalcontaining
dietary supplements. Two recent cases of nephropathy associated with the use
of Chinese botanical preparations, reported in the United Kingdom in July 1999, led to
heightened concern about the presence of aristolochic acid in botanical products used in
the US. A procedure based on an extraction method used by German regulators for the
determination of aristolochic acid in botanical products (1) has been developed and
applied to a variety of botanicals and botanical-containing dietary supplements.
Aristolochic acid is extracted from the sample matrix with aqueous methanol/formic acid.
The concentration of aristolochic acid in the extract is determined by gradient HPLC with
detection at 390 nm and presence confirmed by LCYMS using either an ion trapping mass
spectrometer or a triple quadrupole mass spectrometer.

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