安捷伦1260液相色谱-G6400系列三级四极杆质谱联用仪现场培训手册

仪器资料 · 发表于 2018-6-19 16:45:16

目录
版本说明 .................................................................................................................................................................... 2
目录 .......................................................................................................................................................................... 3
1 基本原理 ................................................................................................................................................................ 5
1.1四极杆质量分析器的基本原理...................................................................................................................................................5
1.2 三级四极杆的工作方式 ...............................................................................................................................................................7
全扫描（MS2 Scan） ................................................................................................................................................ 7
选择离子监测（SIM） ............................................................................................................................................. 7
子离子扫描（Product Ion Scan） ............................................................................................................................. 8
母离子扫描 ............................................................................................................................................................... 8
中性丢失扫描............................................................................................................................................................ 8
多重反应监测（MRM） ........................................................................................................................................... 8
1.3 离子源技术和原理 .......................................................................................................................................................................9
1.3.1 ESI源—大气压电喷雾离子源 ......................................................................................................................... 9
1.3.2 APCI源—大气压化学电离离子源 ................................................................................................................... 9
1.3.3 ESI with Agilent Jet Steam Technology—带有安捷伦喷射流技术的ESI源 .................................................. 10
1.3.4不同离子源的比较 .................................................................................................................................................................10
2 硬件概览 ...............................................................................................................................................................11
2.1典型配置图 ................................................................................................................................................................................11
2.2仪器序列号位置 ........................................................................................................................................................................11
2.4系统结构图示意图 ....................................................................................................................................................................12
3 开机和关机 ............................................................................................................................................................13
3.1 开机步骤 ....................................................................................................................................................................................13
3.2关机步骤 ....................................................................................................................................................................................14
4 软件概览 ................................................................................................................................................................16
5 仪器配置和快捷控制功能 .....................................................................................................................................19
5.1 自动进样器的配置和快捷控制功能 .........................................................................................................................................19
微盘自动进样器G1367、G1377和G4226的Injector配置界面 ........................................................................ 20
标准自动进样器G1329的Injector配置界面 ....................................................................................................... 21
5.2泵的配置和快捷控制功能 ........................................................................................................................................................23
5.3 柱温箱的配置和快捷控制功能 .................................................................................................................................................24
5.4 DAD的配置和快捷控制功能.....................................................................................................................................................25
5.5 VWD的配置和快捷控制功能 ...................................................................................................................................................25
5.6 三级四极杆的配置和快捷控制功能 .........................................................................................................................................26
6 调谐和校正 ...........................................................................................................................................................27
6.1 自动调谐步骤 ............................................................................................................................................................................27
6.2 检验调谐的介绍及使用 .............................................................................................................................................................29
6.3 手动调谐的介绍和使用 .............................................................................................................................................................30
7 方法编辑 ...............................................................................................................................................................32
7.1新建方法 ....................................................................................................................................................................................32
7.2自动进样器的参数设置 ............................................................................................................................................................33
7.2.1 微盘自动进样器的参数设置（G1367，G4226） ........................................................................................ 33
7.2.2 标准自动进样器的参数设置（G1329） ...................................................................................................... 34
7.3 泵的参数设置 ............................................................................................................................................................................35
7.3.1 二元泵的参数设置 ......................................................................................................................................... 35
7.3.2 四元泵的参数设置 ......................................................................................................................................... 36
7.4 柱温箱选项卡 ............................................................................................................................................................................36 一起下吧：www.yqdaw.com
7.5 紫外检测器的参数设置 .............................................................................................................................................................37
7.5.1 DAD检测器的参数设置 ................................................................................................................................ 37
7.5.2 VWD检测器的参数设置 ............................................................................................................................... 38
7.6三级四极杆采集参数的设定 ....................................................................................................................................................39
7.6.1 调谐文件选择、时间过滤和时间分段等设定 ............................................................................................. 39
7.6.2 全扫描（MS2 Scan）采集模式的参数设置 ................................................................................................. 40
7.6.3 选择离子监测（MS2 SIM）采集模式的参数设置 ....................................................................................... 40
7.6.4 子离子扫描（Product Ion Scan）采集模式的参数设置 .............................................................................. 41
7.6.5 多重反应监测（MRM）采集模式的参数设置 ............................................................................................ 41
7.6.6 ESI源的参数设置 ........................................................................................................................................... 42
7.6.7 带有安捷伦喷射流技术的ESI源的参数设置 .............................................................................................. 43
7.6.8 APCI源的参数设置 ........................................................................................................................................ 44
7.6.9 色谱图界面设置 ............................................................................................................................................. 44
7.6.10 Instrument界面设置 .................................................................................................................................... 45
7.7 保存方法 ....................................................................................................................................................................................45
8 运行单针样品 ........................................................................................................................................................46
9 编辑和运行WORKLIST ...........................................................................................................................................47
9.1创建工作列表 ............................................................................................................................................................................47
9.2添加一个样品 ............................................................................................................................................................................47
9.3添加多个样品 ............................................................................................................................................................................48
9.4 保存工作列表 ............................................................................................................................................................................49
9.5 运行工作列表 ............................................................................................................................................................................49
10 定性分析 .............................................................................................................................................................50
10.1打开文件 ..................................................................................................................................................................................50
10.2放大或缩小谱图 ......................................................................................................................................................................51
10.3提取色谱图 ..............................................................................................................................................................................51
10.4提取质谱图 ..............................................................................................................................................................................52
10.5提取和扣除质谱背景 ..............................................................................................................................................................53
10.6改变质谱图离子显示的阈值 ..................................................................................................................................................55
10.7 WALK CHROMATOGRAM和SPECTRUM PREVIEW ................................................................................................................................56
10.8 积分 ..........................................................................................................................................................................................57
10.9 计算信噪比 ..............................................................................................................................................................................58
11定量分析——创建定量的批处理和定量方法 ......................................................................................................59
11.1 内标法定量（ISTD） ...............................................................................................................................................................59
12 仪器的日常维护 ...................................................................................................................................................68
12.1 MS维护周期表 ........................................................................................................................................................................68
12.2 冲洗雾化器组件（NEBULIZER） ................................................................................................................................................68
12.3离子源的清洗和维护 ..............................................................................................................................................................69
12.4 机械泵的日常维护 ...................................................................................................................................................................69
14.添加或更换机械泵油 .................................................................................................................................................................69
13 LC和MS常用消耗品 ...........................................................................................................................................71
13.1 1100/1200/1260系列液相色谱仪常用消耗品 .......................................................................................................................71
13.2 1290系列液相色谱仪常用消耗品 ..........................................................................................................................................72
13.3 质谱常用消耗品.......................................................................................................................................................................72
14. 建立QQQ定量方法的操作流程 .........................................................................................................................73