waters2690分离单元操作指南

仪器资料 · 发表于 2018-6-28 12:09:34

waters2690分离单元操作指南
How to Use This Guide..................................................................... 22
Chapter 1
Introduction to the Waters 2690 Separations Module ...................... 26
1.1 Separations Module Overview.............................................. 27
1.2 Solvent Management System Overview ............................... 29
1.3 Sample Management System Overview............................... 32
1.4 Operating Configurations ...................................................... 36
1.4.1 System Controller Mode ............................................ 36
1.4.2 No Interaction Mode .................................................. 38
1.4.3 Remote Control Mode................................................ 38
1.5 Options and Accessories ...................................................... 40
Chapter 2
Installing the 2690 Separations Module .......................................... 44
2.1 Installation Overview............................................................. 44
2.2 Site Requirements ................................................................ 45
2.3 Firmware and Software Requirements ................................. 47
2.4 Unpacking ............................................................................. 48
2.5 Making Power Connections................................................... 48
2.5.1 Installing/Replacing the Power Supply Fuse.............. 48
2.5.2 Making the Power Supply Connection ....................... 50
2.6 Making Fluidic Connections .................................................. 51
2.6.1 Installing the Detector Drip Tray................................. 52
Table of Contents
一起下吧：www.yqdaw.com
Table of Contents 7
2.6.2 Installing Solvent Supply Tubing ................................ 53
2.6.3 Making Sparge Gas Connections .............................. 54
2.6.4 Installing the Degasser Vent Tubing........................... 56
2.6.5 Making Waste Line Connections ............................... 57
2.6.6 Installing Needle Wash and Plunger Seal Wash
Reservoirs ................................................................. 58
2.6.7 Connecting the Column ............................................. 59
2.6.8 Connecting the Detector ............................................ 60
2.7 Signal Connections ............................................................... 61
2.7.1 I/O Signal Connections.............................................. 62
2.7.2 Digital Signal Connections......................................... 66
2.8 Adding Hardware Options..................................................... 68
2.8.1 Installing an Optional Sample Loop........................... 68
2.8.2 Installing the Column Heater ..................................... 70
2.8.3 Installing a Syringe .................................................... 71
Chapter 3
Preparing the 2690 Separations Module for Operation ................... 72
3.1 Operating Overview .............................................................. 74
3.1.1 Screen Display Overview........................................... 74
3.1.2 Using the Keypad....................................................... 76
3.2 Configuring the Separations Module..................................... 80
3.2.1 Setting Configuration Parameters .............................. 81
3.2.2 Configuring the Operating Mode................................ 91
3.3 Preparing the Solvent Management System ........................ 96
3.3.1 Preparing Solvent Reservoirs .................................... 97
一起下吧：www.yqdaw.com
8 Table of Contents
3.3.2 Degassing Solvents ................................................... 98
3.3.3 Priming the Plunger Seal Wash Pump ...................... 99
3.3.4 Priming the Solvent Management System............... 100
3.4 Preparing the Sample Management System...................... 101
3.4.1 Purging the Sample Management System .............. 101
3.4.2 Priming the Needle Wash Pump.............................. 102
3.4.3 Adjusting the Seal Pack ........................................... 103
3.4.4 Loading Carousels................................................... 104
3.5 Recommended Procedures for Preparing for Operation..... 105
3.6 Powering Off the Separations Module................................. 112
Chapter 4
Front Panel Control ........................................................................ 113
4.1 Routine Startup................................................................... 113
4.2 Loading Sample Vials ......................................................... 114
4.3 Monitoring Your HPLC System............................................ 116
4.4 Performing Direct Functions................................................ 120
4.4.1 Performing a Dry Prime ........................................... 122
4.4.2 Performing a Wet Prime........................................... 124
4.4.3 Purging the 2410 and 410 Reference Cell............... 127
4.4.4 Programming the Sparge Cycles............................. 128
4.4.5 Equilibrating the System.......................................... 129
4.4.6 Conditioning the Column ......................................... 131
4.4.7 Injecting Samples .................................................... 132
Making Automatic Runs ................................................................. 134
5.1 Making Automatic Runs in a Stand-Alone Mode................. 135
5.1.1 Running a Sample Set............................................. 136
5.1.2 Viewing Sample Sets ............................................... 138
5.1.3 Making a Run From a Sample Template.................. 139
5.1.4 Modifying a Sample Set During a Run .................... 141
5.1.5 Stopping a Run ........................................................ 142
5.2 Making Automatic Runs Under Millennium Control............. 142
5.3 Making Automatic Runs Under Micromass MassLynx
Control ............................................................................... 144
Chapter 6
Creating Methods, Sample Sets, and Sample Templates ............. 145
6.1 Creating and Editing Separation Methods .......................... 146
6.1.1 Creating a Separation Method................................. 147
6.1.2 Editing a Separation Method ................................... 148
6.1.3 Copying and Editing a Separation Method .............. 149
6.1.4 Locking and Unlocking a Separation Method .......... 149
6.2 Setting Separation Method Parameters .............................. 150
6.2.1 Setting Mobile Phase Screen Parameter Values ..... 150
6.2.2 Setting Sample Parameter Values ........................... 158
6.2.3 Setting Autosampler Parameter Values ................... 159
6.2.4 Setting Column Parameter Values........................... 161
6.2.5 Setting I/O Parameter Values .................................. 162
6.2.6 Setting Detector Parameters.................................... 166
6.3 Creating and Editing a Sample Set ..................................... 175
10 Table of Contents
6.3.1 Functions ................................................................. 177
6.3.2 Linking Rows in a Sample Set ................................. 180
6.4 Creating a Sample Template............................................... 181
6.5 Using the Disk Drive ........................................................... 182
Chapter 7
Maintenance .................................................................................. 185
7.1 Maintenance Considerations .............................................. 185
7.2 Maintaining the Solvent Management System.................... 187
7.2.1 Overview ................................................................. 187
7.2.2 Removing the Head, Seal Wash Assembly, and
Plunger ................................................................... 190
7.2.3 Replacing the Plunger Seals .................................. 191
7.2.4 Replacing the Seal Wash Assembly Seals .............. 193
7.2.5 Cleaning/Replacing a Plunger ................................ 195
7.2.6 Replacing an Inlet Check Valve Cartridge ............... 197
7.2.7 Replacing the In-Line Filter ..................................... 199
7.3 Maintaining the Sample Management System ................... 201
7.3.1 Overview ................................................................. 201
7.3.2 Replacing the Syringe ............................................. 203
7.3.3 Replacing the Needle and Seal Pack ...................... 207
7.3.4 Cleaning the Sample Compartment ........................ 213
7.4 Updating the Software Version Using the Floppy Disk
Drive .................................................................................. 215
Diagnostics and Troubleshooting ................................................... 216
8.1 Safety and Handling............................................................ 217
8.2 Using the Error Log ............................................................ 217
8.3 Performing Main Diagnostics .............................................. 219
8.3.1 Priming the Plunger Seal Wash Pump .................... 220
8.3.2 Performing the Compression Check ....................... 222
8.3.3 Priming the Needle Wash Pump ............................. 223
8.3.4 Adjusting the Seals.................................................. 224
8.3.5 Service Utilities Diagnostics ................................... 227
8.4 Performing Other Diagnostics ............................................. 227
8.4.1 Performing the Motors and Valves Diagnostic ........ 230
8.4.2 Performing the Sensors Diagnostic ........................ 232
8.4.3 Performing the Static Leak Test .............................. 234
8.4.4 Performing the Valve Leak Test ............................... 236
8.4.5 Performing the Head Removal and Replacement
Utility ....................................................................... 238
8.4.6 Performing the Inputs and Outputs Diagnostic ....... 238
8.4.7 Performing the Keypad Diagnostic .......................... 240
8.4.8 Performing the Display Diagnostic ........................... 240
8.4.9 Performing the Carousel Test ................................. 241
8.4.10 Performing the In-Line Degasser Test .................. 241
8.4.11 Performing the Sample Heater/Cooler Test .......... 242
8.4.12 Performing the Defrost Sample Heater/Cooler
Procedure ............................................................... 244
8.4.13 Performing the Column Heater Test ...................... 245
8.4.14 Rebuilding the Injector Valves ............................... 246
8.4.15 Turning Off the Gradient Proportioning Valve
(GPV) ...................................................................... 247
8.4.16 Creating a GPV Test Method ................................. 247
8.4.17 Performing the Firmware Checksum ..................... 248
8.5 Troubleshooting................................................................... 249
8.5.1 General Troubleshooting Hints................................. 249
8.5.2 Troubleshooting Chromatography Problems ........... 251
8.5.3 Troubleshooting Hardware Problems ...................... 259
Appendix A
Specifications ................................................................................. 266
Appendix B
Spare Parts..................................................................................... 273
B.1 Solvent Management System Spare Parts......................... 273
B.2 Sample Management System Spare Parts......................... 274
B.3 Separations Module Spare Parts........................................ 276
B.4 Vials and Low Volume Inserts............................................. 276
Appendix C
Warranty Information ...................................................................... 279
C.1 Limited Express Warranty................................................... 279
C.2 Shipments, Damages, Claims, Returns.............................. 283
Appendix D
Solvent Considerations................................................................... 285
D.1 Introduction ......................................................................... 285
D.2 Solvent Compatibility .......................................................... 286
D.3 Solvent Miscibility ............................................................... 288
D.4 Buffered Solvents................................................................ 292
D.5 Head Height........................................................................ 293
D.6 Solvent Viscosity................................................................. 293
D.7 Mobile Phase Solvent Degassing....................................... 293
D.7.1 Gas Solubility .......................................................... 293
D.7.2 Solvent Degassing Methods.................................... 294
D.7.3 Solvent Degassing Considerations.......................... 295
D.8 Wavelength Selection ......................................................... 295
Index .......................................................................................... 300

请点击此处下载

请先注册会员后在进行下载

已注册会员，请先登录后下载