Agilent 1200液相色谱仪现场培训教材（适用工作站版本B04.01）
一、 目录
二、 培训目的 .................................................................................................................... 3
三、 培训准备 .................................................................................................................... 3
1. 仪器准备 .................................................................................................................... 3
2. 溶剂准备 .................................................................................................................... 3
四、 方法建立 .................................................................................................................... 3
1. 开机准备 .................................................................................................................... 3
2. 数据采集方法编辑 .................................................................................................... 5
􀀹 G1310A(单元泵) ........................................................................................................ 5
􀀹 G1312A，G1312B XL(二元泵)/G1311A(四元泵) ...................................................... 7
􀀹 G1313A(标准型自动进样器)，G1329A/B(自动控温自动进样器) .......................... 8
􀀹 G1367B/C/D(高性能自动进样器) ............................................................................. 9
􀀹 G1328A(手动进样器) .............................................................................................. 11
􀀹 G1316A,G1316B XL(柱温箱) .................................................................................... 11
􀀹 G1314A/B/D/E,G1314C XL(可变波长紫外检测器) ................................................. 13
􀀹 G1315B/D ,G1315C XL(二极管阵列检测器)/ G1365B/D ,G1365C XL(多波长紫外检
测器) ................................................................................................................................ 15
􀀹 G1362A(示差检测器) .............................................................................................. 17
􀀹 G1321A(荧光检测器) .............................................................................................. 19
􀀹 G4218A(蒸发光散射检测器)................................................................................... 23
3. 数据分析方法编辑 .................................................................................................. 25
􀀹 谱图优化 .................................................................................................................. 25
􀀹 积分参数优化 .......................................................................................................... 25
􀀹 校正 .......................................................................................................................... 27
􀀹 打印报告 .................................................................................................................. 28
􀀹 光谱 .......................................................................................................................... 29
5. 进样 .......................................................................................................................... 35
􀀹 单针进样 .................................................................................................................. 35
􀀹 序列进样 .................................................................................................................. 36
五、 关机 .......................................................................................................................... 38
1. 关闭检测器的灯 ...................................................................................................... 38
2. 冲洗系统 .................................................................................................................. 38
3. 封存色谱柱 .............................................................................................................. 38
4. 关闭电脑 .................................................................................................................. 39
5. 关闭模块 .................................................................................................................. 39
六、 RRLC(快速液相)注意事项 ....................................................................................... 39
七、 Agilent 1200 保养维护 ............................................................................................ 40
八、 Agilent 1200 常见问题解决方法 ............................................................................ 42
1. 仪器准备： Agilent 1200
􀀹 G1310A(单元泵)；G1312A，G1312B XL(二元泵)；G1311A(四元泵)
􀀹 G1313A(标准型自动进样器)；G1329A/B(自动控温自动进样器)；G1367B/C/D(高
性能自动进样器)
􀀹 G1328A(手动进样器)
􀀹 G1316A,G1316B XL(柱温箱)
􀀹 G1314A/B/D/E,G1314C XL(可变波长紫外检测器)
􀀹 G1315B/D ,G1315C XL(二极管阵列检测器)
􀀹 G1365B/D ,G1365C XL(多波长紫外检测器)
􀀹 G1362A(示差检测器)
􀀹 G1321A(荧光检测器)
􀀹 G4218A(蒸发光散射检测器)
􀀹 色谱柱：Zorbax Eclipse XDB-C18 150 x 4.6 mm, 5um column (P/N 993967-902
/5063-6600)
2. 溶剂准备：
􀀹 色谱级纯或优级纯乙腈或甲醇。
􀀹 二次蒸馏水
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