Preface .............................................................................................................v
Contributors .....................................................................................................xi
PART I. PERFORMING AND OPTIMIZING PCR
1 Polymerase Chain Reaction: Basic Principles and Routine Practice
Lori A. Kolmodin and David E. Birch .................................................. 3
2 Computer Programs for PCR Primer Design and Analysis
Bing-Yuan Chen, Harry W. Janes, and Steve Chen ........................ 19
3 Single-Step PCR Optimization
Using Touchdown and Stepdown PCR Programming
Kenneth H. Roux .................................................................................. 31
4 XL PCR Amplification of Long Targets from Genomic DNA
Lori A. Kolmodin .................................................................................. 37
5 Coupled One-Step Reverse Transcription and Polymerase Chain
Reaction Procedure for Cloning Large cDNA Fragments
Jyrki T. Aatsinki ................................................................................... 53
6 Long Distance Reverse-Transcription PCR
Volker Thiel, Jens Herold, and Stuart G. Siddell ............................. 59
7 Increasing PCR Sensitivity for Amplification
from Paraffin-Embedded Tissues
Abebe Akalu and Juergen K. V. Reichardt ....................................... 67
8 GC-Rich Template Amplification by Inverse PCR:
DNA Polymerase and Solvent Effects
Alain Moreau, Da Shen Wang, Steve Forget, Colette Duez,
and Jean Dusart............................................................................... 75
9 PCR Procedure for the Isolation of Trinucleotide Repeats
Teruaki Tozaki ...................................................................................... 81
10 Methylation-Specific PCR
Haruhiko Ohashi .................................................................................. 91
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